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Poster Presenters

Palinee Detsomboonrat,PhD

Associate Professor
Chulalongkorn University

Dr. Palinee is Associate Professor in Dental Public Health, ,at Chulalongkorn University. She was conferred Doctor of Philosophy (PhD) in Dental Public Health and Master of Science in Pediatric Dentistry and Doctor of Dental Surgery (DDS) from Chulalongkorn University, Thailand. She is a Fellow of Royal College of Dental Surgeons of Thailand (FRCDT) in Dental Public Health. She has been involved in several workshops and projects under the Ministry of Public Health related to improving oral health in children. Her research interests focus on caries management, fluorides, quality of life, and community dental care. Dr. Palinee is currently working as an associate Professor in Dental Public Health, the Department of Community Dentistry at the Faculty of Dentistry, Chulalongkorn University. She is also a committee for the Master of Science and Doctor of Philosophy (PhD) programs in Dental Public Health.


Evaluating the Impact of Light Curing on Silver Diamine Fluoride: Clinical Efficacy and Professional Perceptions
Empirical evidence supports the effectiveness of silver diamine fluoride (SDF) in arresting caries on both primary and permanent teeth in clinical settings. It has also been shown to enhance the microhardness and mineral density of carious lesions in vitro. A recent systematic review highlighted that studies using SDF with light curing in vitro and ex vivo may improve SDF’s properties, including its penetration potential, silver ion precipitation, dentine hardness, and antimicrobial activity. However, there is no clinical evidence to suggest that light curing offers additional benefits in arresting or preventing dental caries when used with SDF.

Given this uncertainty, it is essential to explore dental professionals’ perceptions of SDF use, both with and without light curing, to identify facilitators and barriers to its application. A qualitative study was conducted with dental professionals who had experience using SDF in both methods. Participants generally favored SDF with light curing, citing perceived benefits such as faster treatment and improved moisture control. Nonetheless, some expressed concerns, either due to current practice guidelines or the lack of robust evidence supporting the efficacy of light curing.

A comparative study examining the effectiveness of semi-annual applications of 38% SDF with and without light curing in children aged 5-7 years with active dentine carious lesions found no significant difference in caries arrest rates between the two groups (p=0.161). These findings suggest that adding light curing to SDF-treated teeth does not significantly enhance its caries-arresting ability in primary teeth.

To establish the long-term clinical effects of SDF with light curing compared to conventional methods, further research is required. Additionally, studies focusing on the cost-effectiveness of these two approaches and their impact on patient or parent adherence would be valuable.

Zhiyan Jin, MS

Graduate Student
Seoul National University

Zhiyan Jin is currently pursuing a master’s degree in prosthodontics at Seoul National University School of Dentistry. Her research focuses on dental implants, utilizing advanced computer simulation techniques to study implant performance and impact. She plans to investigate how implant design and preload affect bone during placement. Zhiyan’s primary research is implantology. She aims to seek further knowledge into this field. Before entering the graduate program, she earned her bachelor’s degree from YanBian University in China, where she developed a strong interest in prosthodontics during her five years of study, which ultimately shaped her current research direction.


Biomechanical effects of implant placement in the maxillary anterior region with bone loss: A 3D finite element analysis

Zhiyan Jin1,Mi-El Kim2, Mary Delia Z. Bondoc1, Ho-Beom Kwon1
1Dental Research Institute and Department of Prosthodontics, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
2Dental Research Institute and Department of Oral Anatomy, School of Dentistry, Seoul National University, Seoul, Republic of Korea.

OBJECTIVES:
The purpose of this FEA study was to compare and evaluate the effects on bone of two treatment methods: implants placed in the basal bone or implants with a narrow diameter in the maxillary anterior region with a thin ridge state.
MATERIALS AND METHODS:
Two models to be implanted in the anterior area where the ridge was thinned due to bone absorption were selected. The model to be implanted in the basal bone has a design of (Ø 3.2 X 7 X (6)). The other model has a mini implant (Megagen Mini (Ø 3.3 X 13 X (6)). Two models were assigned as Model 1 and Model 2. Models were created using a mesh generation program (Visual-Mesh; ESI Group). A 45-degree oblique load (300 N) to the axis of the implant was applied on the occlusal surface of the crown for the models with occlusal load.
RESULTS:
Model 1 and Model 2 showed similar stress distributions and displacement values. When comparing the results of each component, the results were similar. In the results of Model 1, where the implant was placed in the basal bone, the stress level of the screw increased, but the stress levels of the cortical bone and the cancellous bone showed relatively small fluctuations.
CONCLUSIONS:
In the maxillary anterior region where bone resorption has progressed, treatment can be performed with Model 1, which was inserted into the basal bone that is not resorbed, instead of the existing method. Since implants are placed into the basal bone, complications such as peri-implantitis may not occur, so it is expected to be a good treatment method.

Lin Xu, DDS, PhD

Graduate Student
Osaka University

I was born in Henan, China, a place rich in history as an ancient capital and famous for food like noodles, red dates and soup dumplings. I was raised by loving and encouraging parents. After high school, I moved to Sichuan due to my parents’ work, a region known for pandas and spicy food. In 2015, I started my bachelor’s in dentistry at Sichuan University. I’ve always wanted to experience different cultures, and Japan was a dream country for me. So, in 2020, I came to Osaka University to pursue my PhD. I feel truly lucky to have joined this department—I’ve had amazing support and help from my supervisor, advisor, and lovely lab members. And I’m deeply grateful for their help throughout my PhD journey.


Diminished retinoid signaling transforms the murine frontonasal mesenchyme into maxillary components.
Lin Xu, Yanran Wu, Takayuki Tsujimoto, Qi Wang, Xiuping Nie, Daisuke Motooka, Maiko Sugihara, Toshihiro Inubushi, Masaya Yamaguchi, Lisa Sandell, Trainor Paul, Takashi Yamashiro, Hiroshi Kurosaka
Vertebrate facial morphogenesis depends on the correct specification of embryonic facial prominences, which requires the accurate acquisition of positional identity by cranial neural crest cells (CNCCs). This positional identity is instructed by environmental signals that regulate transcription factor expression during later developmental stages. Retinoid signaling plays a critical role in facial development as a transcriptional regulator, and its disturbance results in wide spectrum of facial defects, including orofacial cleft. In this study, we identified retinoid signaling as a critical molecule for establishing frontonasal process (FNP) identity in post-migratory CNCCs. During early murine craniofacial development, retinoid signaling is specifically active in the FNP. In retinoid signaling deficient mutants, we observed the loss of FNP-derived structures, such as upper incisors and nasal cartilage, along with the formation of ectopic tissues, including abnormal protrusions and bone in the frontonasal region. Single cell RNA-seq revealed that retinoid signaling – targeted CNCCs in the FNP were transformed into the maxillary CNCCs in mutant embryos. Multiomic profiling via single nucleus RNA-seq and single nucleus ATAC-seq further uncovered that retinoid signaling represses key transcription factors (LHX6, MEIS2, and SIX1) in a CNCC subpopulation of the FNP, which is responsible for specifying murine FNP and maxillary process identities. These findings suggest that early embryonic retinoid signaling is essential for establishing frontonasal and maxillary process identities required for proper facial morphogenesis.

Sujung Choi,PhD

Postdoctoral Researcher
Seoul National University

Education
・Ph.D. – Department of Oral Pathology, School of Dentistry, Seoul National University
・MPH – International Public Health, Yonsei University
・B.S. – State University of New York
Grant
・Principal Investigator: Ministry of Science and ICT – Mid-Career Researcher Support Program
・Project Title: “Investigation of signaling pathways involved in mitotic catastrophe induction and discovery of new anti-cancer agents for head and neck cancer”
・Grant Period: September 1, 2022 – February 29, 2025
・Grant Amount: ₩250,000,000
・Focus: Explore signaling mechanisms inducing mitotic catastrophe and identify therapeutic agents for head and neck cancers.
・Principal Investigator: Ministry of Science and ICT – Creative Challenge Research Support Program
・Project Title: “Efficacy of Pseudolarix kaempferi extract in inducing apoptosis in head and neck cancer cells”
・Grant Period: June 1, 2020 – May 31, 2022
・Grant Amount: ₩100,000,000
・Choi, S. J., Swarup, N., Shin, J. A., et al. (2022). “Myeloid cell leukemia-1 expression in cancers of the oral cavity: a scoping review.” Cancer Cell International, 22(1), 182.
・Choi, S. J., Ahn, C. H., Hong, K. O., et al. (2021). “Molecular mechanism underlying the apoptotic modulation by ethanol extract of Pseudolarix kaempferi in mucoepidermoid carcinoma of the salivary glands.” Cancer Cell International, 21(1), 427.
・Ahn, C. H., Oh, K. Y., Jin, B., et al. (2023). “Targeting tumor-intrinsic PD-L1 suppresses the progression and aggressiveness of head and neck cancer by inhibiting GSK3β-dependent Snail degradation.” Cellular Oncology, 46(2), 267–282


Triggering mitotic catastrophe by podophyllotoxin induces apoptosis in oral squamous cell carcinoma
Objective: Mitotic catastrophe (MC) is characterized by abnormal nuclear morphology and incomplete cell division due to aberrant mitosis, leading to various forms of cell death, including apoptosis. This study aimed to investigate the relationship between MC and apoptosis in oral squamous cell carcinoma (OSCC) using Podophyllotoxin (PPT), a natural compound with antimitotic properties.
Methods: The effects of PPT on OSCC cells were systematically examined, focusing on cell proliferation, cell cycle progression, and morphological changes associated with MC. DNA damage, polyploidy, cell cycle arrest, and apoptosis-related protein activation were assessed in both 2D and 3D HSC-3 cell models.
Results: PPT induced DNA damage, leading to cell cycle arrest, polyploidy, and MC, followed by apoptosis. Caspase-2 and t-Bid activation were crucial in linking MC to apoptosis. The effects were consistent across both 2D and 3D models, suggesting a robust therapeutic potential.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning [NRF-2022R1A2C1091608 and RS-2023-00247502].

Sirikarn Arunyanak, MSc

Associate Professor
Chulalongkorn University

RESEARCH INTEREST
Periodontology and Dental Implant
EDUCATION
2014 Postdoctoral fellowship, University of Louisville, Louisville, United States
2012 Diplomate, The Royal College of Dental Surgeons of Thailand, Bangkok, Thailand
2011 Master of Science (Periodontics), Chulalongkorn University, Bangkok, Thailand
2004 Doctor of Dental Surgery (First Class Honor), Chulalongkorn University, Bangkok, Thailand
PROFESSIONAL EXPERIENCE
2022 – Present Academic Committee, The Dental Association of Thailand
2016 – Present Subcommittee, The Dental Association of Thailand
2016 – Present ITI Siam Square Study Club Director


Key Insights from Dental Implant Surveys: Understanding the Prevalence and
Risk Factors of Peri-Implantitis

Peri-implantitis, a significant complication affecting dental implants, has garnered increasing attention in recent years due to its potential to compromise long-term implant success. Surveys conducted globally have provided valuable insights into the prevalence, risk factors, and management strategies associated with peri-implantitis. This presentation aims to summarize key findings from dental implant surveys, highlighting the prevalence of peri-implantitis, the most common patient-related and implant-related risk factors, and patient satisfaction. By understanding these insights, clinicians can better identify at-risk patients, implement effective preventive measures, and improve overall implant longevity.

Su-Min Cho, PhD

Postdoctoral Researcher
Seoul National University

EDUCATION
2019. 03 ~ 2022. 08: Ph.D. (Integrated Program) in Applied Life Science, Yonsei University
2014. 03 ~ 2018. 02: Bachelor’s Degree in Dental Technology, Shingu University
EXPERIENCE
2022. 08 ~ Present: Postdoctoral Researcher, Dental Research Institute, Seoul National University Industry Foundation
2018. 07 ~ 2019. 02: Researcher, Medical Division, Yonsei University Health System Industry Foundation
2017. 02 ~ 2017. 08: CAD/CAM Specialist, Hansol Dental Lab
Certification
2017. 02 Dental Technician, Korea Health Personnel Licensing Examination Institute
PUBLICATIONS
<SCI>
・Trueness, physical properties, and surface characteristics of additive-manufactured zirconia crown. Journal of the Mechanical Behavior of Biomedical Materials. 2024;154:106536.
・Trueness and surface characteristics of 3-dimensional printed casts made with different technologies. The Journal of Prosthetic Dentistry. 2023.
・Comparative assessment of marginal and internal gaps of cast-free monolithic zirconia crowns fabricated from 2 intraoral scanners. J Prosthet Dent. 2022 Aug 16:S0022-3913(22)00431-0.


Comparison of fit, mechanical properties and surface characteristic of subtractive and additive zirconia crown
This study introduces various zirconia 3D printing materials and technologies and investigates mechanical properties such as fit, hardness, and shear bond strength of prosthetics produced using these methods. It also examines surface characteristics to better understand the physical and mechanical behavior of additive manufacturing (AM) processes.

Zirconia crowns were producedu sing both AM and subtractive manufacturing (SM) techniques. The AM groups included four digital light processing (DLP) principles and one stereolithography (SLA) principle, each with different slurry delivery and light-curing methods. The marginal and internal gaps (axial, line angle, occlusal) between crowns and abutments were measured using the replica
technique. Hardness and shear bond strength were evaluated, and surface characteristics were analyzed with scanning electron microscopy (SEM) after printing and sandblasting.

Marginal fit analysis showed the milling group had a marginal fit of 48.45 µm, while AM groups ranged from 42.83 to 81.95 µm, with significant differences between groups (P<0.001), but all within the clinical acceptance range of 120 µm. Vickers hardness for the milling group was 1473.87 HV, while the AM groups ranged from 1441.94 to 1532.53 HV, with significant differences (P<0.001). Shear bond strength was 7.97 MPa for the milling group and 6.97 to 8.97 MPa for the AM groups, with no significant differences. SEM analysis revealed agglomerated zirconia particles, with grooves observed after sandblasting.

Zirconia crowns produced by both AM and SM methods demonstrated clinically acceptable marginal fit and hardness above 1200 HV. Some AM groups showed higher hardness and shear bond strength than the milling group. The varied properties of different zirconia 3D printing technologies suggest their selective use based on clinical needs.

Masayoshi Morita, DDS

Graduate Student
Osaka University

2021-present Ph.D. Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
2014-2020       D.D.S. Osaka University Faculty of Dentistry


A novel host-pathogen interaction related to Fusobacterium nucleatum infection
Fusobacterium nucleatum (F. nucleatum), an oral commensal bacterium, induces the production of inflammatory cytokines at the site of infection. Some reports indicate that F. nucleatum is associated with various systemic diseases and has been observed to colonize ectopically in the intestinal tract. However, the precise mechanism by which F. nucleatum induces inflammation remains unclear. In this study, we focused on the relationship between F. nucleatum and GBPs, one of the interferon-inducing genes to elucidate how F. nucleatum induces IL-1β.
The human monocytic leukemia cell line THP-1 was infected with F. nucleatum at a multiplicity of infection of 100. After infection, cells and supernatants were analyzed by qPCR and ELISA. IL-1β production increased during F. nucleatum infection and was further enhanced by IFN-γ stimulation. To clarify the role of GBPs, GBP knockout macrophages were used. Low levels of IL-1β production were observed in the deficient macrophages.
Western blot analysis revealed GBP1 expression, with an additional band at twice the molecular weight in F. nucleatum-infected and IFN-γ-stimulated cells, suggesting GBP1 dimerization. For further validation, infection experiments were performed with compouns that promote dimerization. Promotion of GBP1 dimerization was observed to result in a significant increase in IL-1β production. Among the GBP family, only GBP1, GBP2, and GBP5 can dimerize. ELISA data from GBP-deficient cells showed reduced IL-1β production specifically in GBP1-deficient cells. Some studies suggest that GBP dimerization activates inflammasomes by recognizing intracellular LPS and activating caspases. This study suggests that F. nucleatum-induced IL-1β production may depend on inflammasome activation via GBP1 dimerization. This finding provides a potential target for therapeutic intervention in diseases in which F. nucleatum is implicated.

Thitaya Chaiboonyarak, DDS, MSc

Lecturer, Committee member of Geriatric Dentistry and Special Patients Care International Program
Chulalongkorn University

Thitaya Chaiboonyarak, DDS, MSc, is a dentist and researcher dedicated to improving patient care, particularly for geriatric and special-needs populations. After earning her Doctor of Dental Surgery with first-class honors from Chiang Mai University in 2013, she completed an MSc in Geriatric Dentistry and Special Patients Care at Chulalongkorn University in 2022. Thitaya has contributed to research at the Excellence Center in Genomics and Precision Dentistry, specifically focusing on oral microbiome profiles in patients with X-linked agammaglobulinemia. She is also deeply committed to building multidisciplinary teams to provide holistic care for special-needs patients, including geriatric and genetic populations. As a consultant on Thailand’s health systems and vulnerable population research, Thitaya plays a key role in guiding projects that address health inequalities, particularly in urban settings, with a strong focus on improving health outcomes for vulnerable communities.


Pioneering Multidisciplinary Care in Special Care Dentistry at Chulalongkorn University
The growing need for specialized dental care in geriatric and special needs populations has driven the development of a multidisciplinary approach. Chulalongkorn University has developed a model that unites experts from the Faculty of Dentistry, Faculty of Medicine, and other stakeholders to address complex dental and systemic challenges. This abstract presents the development and current status of the multidisciplinary Special Care Dentistry team, with a focus on education, research, clinical services, and societal contributions. Educationally, a curriculum integrating lectures, clinical practice, and rotations enhances student proficiency in Special Care Dentistry through collaboration between faculties. In research, partnerships with the Center of Excellence for Medical Genomic and the Center of Excellence in Genomics and Precision Dentistry have advanced research in genomics and dental care for genetic patients. Clinically, Chulalongkorn initiated the Genomic Care Collaborative Clinic (GCC), Thailand’s first multidisciplinary team providing holistic care for genetic patients and families, in cooperation with the Excellence Center for Genomics and Precision Medicine. Societal collaboration with the Urban Studies Institute aims to influence national health policy, addressing health inequalities in urban populations and leveraging expertise in Social Determinants of Health to inform policy development. The program’s partnerships across academic disciplines and institutions have expanded research output and embedded clinical rotations into the curriculum. The GCC clinic now serves as a model for holistic genetic care, while collaboration with the Urban Health Institute continues to shape healthcare policy for vulnerable populations. The multidisciplinary approach at Chulalongkorn University has led to innovation in education, research, and clinical services. However, there is still room for improvement, and ongoing efforts will contribute to future growth and evolving healthcare policies for vulnerable populations.
Keywords: special care dentistry, multidisciplinary team, geriatrics, genetic, healthcare policy, vulnerable populations

Byeong-Min Lee, DDS, PhD

Graduate Student
Seoul National University

Education
2006.03. ~ 2011.2. B.S., School of Chemical and biological engineering, Seoul national university
2011.03. ~ 2021.2. D.D.S.-Ph.D. program, School of Dentistry, Seoul national university
Representative Publications
Lee, B. M., Park, J. W., Jo, J. H., Oh, B., & Chung, G. (2022). Comparative analysis of the oral microbiome of burning mouth syndrome patients. Journal of Oral Microbiology, 14(1), 2052632.
Byeong-Min Lee*, Chisong Lee*, Shayan Fakhraei Lahiji, Ui-Won Jung , Gehoon Chung#, Hyungil Jung#, Dissolving Microneedles for Rapid and Painless Local Anesthesia. Pharmaceutics (2020) 12(4):366.
Byeong-Min Lee*, Hyun-Jung Jo*, Giyeon Park, Yong Ho Kim, Chul-Kyu Park, Sung Jun Jung, Gehoon Chung#, Seog Bae Oh#, Extracellular ATP induces calcium signaling in odontoblasts. Journal of Dental Research (2017) 96(2):200-207.


Oral Microbiome analysis of Temporomandibular Disorder patients: Associations with
clinical symptoms and disability levels

Authors: Byeong-min Lee1, Jiwoon Park1,2,3
affiliation:
1Department of Oral Medicine, Seoul National University Dental Hospital, 101 Daehak-ro, Jongno-gu, Seoul, Republic of Korea
2Department of Oral Medicine and Oral Diagnosis, Seoul National University School of Dentistry, 101 Daehak-ro, Jongno-gu, Seoul, Republic of Korea
3Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, Republic of Korea
Background Temporomandibular disorders (TMD) are complex conditions primarily characterized by mild to moderate pain. While prior research has examined the link between pain disorders and the oral microbiome, the specific role of the microbiome in TMD pathophysiology remains largely unexplored. This study aims to investigate the association between microbial profiles and clinical variables in TMD, hypothesizing that distinct microbial patterns correlate with pain characteristics and specific TMD diagnoses.
Methods The study included 130 TMD patients diagnosed using the Diagnostic Criteria for TMD
(DC/TMD). Participants completed questionnaires on psychological and general health, and whole saliva samples were collected for microbial analysis. The 16S rRNA gene sequences were processed using QIIME 2 to analyze microbial communities. Operational Taxonomic Units were identified, and both alpha and beta diversity metrics were calculated. Comparative analyses correlated microbial diversity with clinical parameters, including pain chronicity and TMD sub-diagnoses, using PCoA, Kruskal-Wallis H tests, and LEFSe analysis.
Results The cohort comprised 105 females and 25 males, with an average age of 37.63 ± 14.4
years. Based on the Graded Chronic Pain Scale, 36 participants had high disability and 94 had low disability. The sample included 70 myalgia, 72 arthralgia, 29 TMD-associated headache, 95 disc displacement, and 63 osteoarthritis patients. Significant differences in alpha diversity were found
between high and low disability groups, with notable associations between microbiome profiles and specific TMD subtypes.
Conclusions This study suggests a potential link between oral microbiome composition and TMD
symptoms, indicating that specific microbial profiles may correlate with the severity and type of TMD manifestations. Despite limitations due to its cross-sectional design, these findings highlight the need for future longitudinal studies to explore causal relationships and the microbiome’s role
in TMD progression.